Warrior's Journal (My POIS Protocol)

[Warrior]

3) Some trouble with Omega-3 supplementation has slowed me down. Gave me anxiety, brain fog, and irritability, so I had to stop everything and figure out what was causing it. Initially I was worried that the effectiveness of Nanna1s POIS Cascade stack was dwindling. Nope, turned out to be an Omega-3 supplement I had recently begun taking for the POIS Cascade stack. Now I just eat regular sardines with extra virgin olive oil in some apple cider vinegar frequently for the Omega-3. And back to feeling amazing, POIS free.

I might be experiencing the same symptoms as you with omega-3 supplements. I'm struggling to find information about this on the internet. Do you have any clue about what causes these symptoms and why it's different with regular fish ?

I fixed it with vitamin D3! My levels must of been getting very low and were causing symptoms with omega3 supplementation and even B vitamins. Try D3, make sure you take it with a fatty meal for absorption. 100mcg of K2 per 1000 D3 to be safe from calcification my specialist said. You could do a temporary megadose phase of 14,000 iu/1.4mg of K2 then drop to 7000 iu/700mcg of K2. Not a doctor but these are rough recommendations from my specialist.

[Warrior]

Thanks for this comprehensive answer, Warrior !
I see that a site that I order from, Vitacost, has introduced a new monolaurin preparation in its brand line.   They have put some inosine in it as well. I took a look to see the utility, and inosine is supposed to also have antiviral activity, and inosine also reduces the level of some pro-inflammatory cytokines like IL-6, which are causing many symptoms in viral infections like influenza.
Would have been very useful to me 25 years ago, when I had mononucleosis/EPV - been very very sick for 6 weeks.

Ah yes, my current supplement is just monolaurin and calcium, but they've added calcium as a filler, so I also have another supplement in the mail for 100% monolaurin. Do you suspect any kind of viral or infection element behind your POIS? I know Nanna1 was big on this theory when he was active on the forum.

No, I do not think that a viral infection in particular is an essential factor in my POIS.  There is no Lyme around here ( too cold, just appeared a few years ago in the southern part of Quebec province, and I am North).  I had EBV ( mononucleosis) in my 30s but already had POIS for over 15 years ( since puberty).  I had lots of colds, flus, allergies, and respiratory tract infections as a child - I was always sick, all year round.  I had dozens of antibiotic treatments.  So, probably my overall poor health is an important factor, but it would be hard to pinpoint one infection out of the hundreds I had.  My immune system was surely not functioning normally. But when I got older and did what I had to do to improve my health, I stopped to have all those viral and bacterial infections, but POIS stayed, even if my immune system got better.

Interesting, thanks for sharing.

[Warrior]

Tested negative for HHV-1, HHV-2, EBV (HHV-4), CMV (HHV-5). Tested positive for VZV (HHV-3) and HHV-6.

Monolaurin is said to be effective on literally all those viruses I tested negative for lol.

Monolaurin continues to work. I'm not sure how realistic it is to take long-term tho. It is a natural antibacterial, and may wipe away the good guys as well. According to Lauricidin's website (original brand behind Monolaurin), it is safe to build up to dosages of 9g/d. Monolaurin also has 4100 reviews on Amazon, with a tonne of positive reviews, an average of 4.6/5. The supplement is regarded as fairly safe, and I would suspect a lot more negative reviews, concern, and conservativeness from the brand if it were truly dangerous in a significant capacity. But I do still worry about long-term use and wiping away good bacteria. Here's a link to official website & dosage: https://www.lauricidin.com/learn/intake-instructions

I notice that I need to take upwards of 2-3g or so/d spread throughout to have high relief of food sensitivities. Any less and it gets a little hit or miss with subtle symptoms. 3g/d gives some pretty strong herx reactions which is why people build up over time slowly, using 30mg pellets (available from the original Lauricidin brand). Even 1g/d still has pretty amazing effects of reducing symptoms tho, the 2-3g/d is for perfect alleviation of all symptoms, even while eating gluten! Note: my food sensitivities are tied into POIS, so when something is effective for my food sensitivities, it is equally effective for actual POIS. Nowdays I mostly do my POIS testing on food sensitivity.

PS: I am going to summarise & share all of my blood work at some point, in a clear organised manner, so I can add it to the medical data listed here at POIS Center.

[Warrior]

Summary of POIS Symptom Reduction & Cure Strategy

This is a WIP summary of my POIS symptom reduction & cure strategy. Much obviously inspired by Nanna1. Some extras from the recommendation of my functional medicine specialist. I believe the root cause of POIS is some kind of infection or gut dysbiosis. My killing/immune supplements are aimed at that.

Note: Yes, it's a decent amount of supplements. Regular CBC and LFT blood work is advised to stay on top of everything. Much of this has also been vetted by my specialist. Any supplements taken should be thoroughly researched, and double-check potential supplement interactions.

Modified Nanna1 POIS Cascade Stack that includes methyl-buffers:
--SAM-e 200mg active 1x (skip until methyl buffer system is working)
--B complex 150 Forte (B12, Folate & B6 are most important as SAM-e cofactors. I prefer a good B complex.)
--AlphaGPC 600-1200mg
--Vitamin D3/K2 7000iu with 700mcg K2
--Omega-3 DHA & EPA 1-2gs

Methyl buffer system
--Vitamin A 3000mcg (only necessary if you're deficient in Vitamin A / need to be careful of Vitamin A toxicity)
--Glycine 3-9g

Use when over-methylated - mops up excess methyl-groups
--Niacin 50mg every 2 hrs until flushing

Killing/Immune:
--Monolaurin, titrate up to minimum 3g/d for 3 months. Don’t want to risk antibiotic resistance if it’s killing the right things. Titrate down if herx reaction is too strong. Dosages up to 9g/d are safe according to Lauricidin’s website.
--Propolis 1-2 pills

May be cycled according to immune stimulation. From Nanna1's Immune Competence Therapy:
--AHCC 1-4 pills
--Beta-glucans 1g
--Andrographis 100mg (do not exceed, Monolaruin is already a potent antibiotic)

--Liposomal Vitamin C 1-2g
--Zinc 50mg
--Quercetin 2g
--Magnesium 2-3 pills (always before bed)

--Mega IgG2000 (biotoxin binder – take with food) 2-4x

*Make sure you are pissing, shitting, and sweating for detoxification.

Probiotics (taken alongside Monolaurin, putting the good guys back in):
--MegaSpore probiotic 1-2
--Raw fermented sauerkraut, kimchi, or kefir (at least 2)

Intravenous Vitamin C:
--Vitamin C drip 15g 4 days consecutively
--Copper 2mg

Other:
--NAC (excellent for immune support but makes me anxious)

Medical testing in the pipeline:
--GI Map (or any top-quality gut microbiome testing - still need to research)
--Potentially more viral and bacteria testing

Original Animal-Based Method (still completely effective):
--Animal-Based Diet by Paul Saladino
--Raw minced garlic
--Fenugreek
--Red meat
--Eggs
--B Complex

Haven’t tested yet, but may be useful:
--Creatine (especially for SAM-e/methylation)
--Mushrooms (Chaga, Cordyceps) from Oriveda, Vibe, or Real Mushrooms (mushroom supplements must be purchased from reliable high-quality brands)
--Vegan diet to reduce AA in POIS Cascade
--Herpes viruses deplete TMG, Taurine, NAD+, & Creatine
--Vitamin E
--Boron
--Schisandra (for kidney support)

[The One]

Awesome work!

It looks like you're combining both of Nanna 1's stacks together.

Is that providing you with better results than the immune stack alone?

PS - Would you mind sharing who's the specialist you're working with? It seems you got this stuff on lock haha

[Warrior]

Awesome work!

It looks like you're combining both of Nanna 1's stacks together.

Is that providing you with better results than the immune stack alone?

PS - Would you mind sharing who's the specialist you're working with? It seems you got this stuff on lock haha

The POIS Cascade Stack has really been the only thing of Nanna1s work that has given me complete immediate relief of POIS symptoms (and food sensitivities). That lasted for 30 days, until it stopped working. I think it stopped working as my D3 levels plummeted (I basically stopped taking it), and I assume some tolerance to the SAM-e was developed. I recently added CLA for the first time, and do believe this has had a positive impact, although I do admit I started it just after having success with Monolaurin, so it's a bit difficult to tell how much it's helping. Regardless, SAM-e, D3, and B vitamins have all significantly improved my POIS, even to this day, so I continue to take the Cascade Stack. His ICT pre-pack didn't do much, although I didn't extensively test it. I haven't tried his betaherpes pre-pack, though I am curious to how I would respond to that.

And yes, the specialist I'm working with is Oscar Sierra from Sierra Collaborative Medicine. He works predominantly with cancer patients and rare diseases. I do online consultations with him. Totally recommend! I would see him more regularly, but the price of sessions add up quickly. Nonetheless, he's been a big help thus far in ruling out potential causes, and was also the one who recommended me personally the Monolaurin, which I've had great success with so far. With that being said, I don't know how the monolaurin will work long-term, and I don't know if it's advised to take it permanently.

[Warrior]

I went without vitamin D3 for a few days as I ran out of K2. And boy did I feel the negative effects of it, slowly creeping in day by day. For me it increases auditory thought noise, which is like an undertone of anxiety. I think vitamin D3 is really important for POISers. I think that's been well-established here already, so just re-emphasising that. When you have an inflammatory condition like ours, it probably drains these vital resources like D3 a lot quicker.

I take 7000iu/d, ideally with 700mcg of K2. Maybe a bit overkill on the K2, but my specialist recommended me 100mcg per 1000iu D3 to be completely safe in regards to the calcification risk.

[Warrior]

Hypothesis: Nanna1s method stopped working 100% after 30 days due to over-methylation from SAM-e. The method has now been working perfectly as of 06/01/2024 since I integrated these changes:

Over-methylation can create high levels of dopamine, norepinephrine, and epinephrine in the brain. Norepinephrine stimulates arachidonic acid (AA) release, the inflammatory fatty acid involved in the POIS Cascade.

Anecdotal: Nanna1's POIS Cascade method worked perfectly for a month until it's effectiveness waned. Initially, I was taking the 200mg active SAM-e once daily, then titrated up to 2x as per instructions, despite feeling like I didn't always need the 2nd dosage. I also noticed that after taking SAM-e or TMG, I would get a very distinct episode of body aches, as if it had triggered a tiny episode of POIS symptoms. This did not happen when I first began taking the SAM-e. I believe this change began happening once I started to become over-methylated from excess SAM-e. Taking methyl buffers should theoretically fix this problem:

Modified Nanna1 POIS Cascade Stack that includes methyl-buffers:
--SAM-e 200mg active 2x (Skip until you are no longer over-methylated. See notes below for individual dosage)
--B complex 150 Forte (B12, Folate & B6 are most important as SAM-e cofactors. This B complex makes me feel good so I take it and is probably the reason why I don't need as much SAM-e.)
--Choline source 600-1200mg (I personally haven't noticed any difference in supplementing choline, but it certainly is an important nutrient for your methylation cycle. I do however feel amazing after eating eggs.)
--Vitamin D3/K2 14,000iu with 1.4mg K2 (Very important, see my post here.)
--Omega-3 DHA & EPA 1-2gs (I've noticed zero effect from supplementing Omega-3, but I take it as it's generally agreed upon that Omega-3 supplementation is good for you. It's spoken about in the POIS Cascade, but according to Nanna1 is only useful in this context if you eat a low AA vegan diet)

Methyl buffer system
--Vitamin A (Only necessary if you're deficient in Vitamin A. Warning: Vitamin A toxicity is a real concern with large dosages. I personally have not been able to tolerate retinol supplementation for some strange reason, so I will be experimenting with eating liver. Cod liver oil would be a great source.)
--Glycine 3-9g (Best taken before bed as it can make you sleepy. Magnesium glycinate may be an effective way of supplementing both.)

Use when over-methylated - mops up excess methyl-groups
--Niacin 50mg every 2 hrs until flushing

Extra notes:
--Stop SAM-e and TMG supplementation until methyl buffer system is working and/or you are no longer over-methylated.
--Make sure you have adequate levels of iron. Most people are not iron deficient so I did not include it in the list of supplements.
--Amount of SAM-e you need to supplement is probably going to vary on an individual basis. Currently testing 400mg morning & night after a period of daily Glycine/Vit A.

Helpful resources:
https://www.reddit.com/r/MTHFR/comments/169595o/overundermethylator_or_deficient_methyl_buffering/
https://butternutrition.com/signs-of-overmethylation/

*If the stack stops working in the future, look no further than something becoming unbalanced in the methylation cycle.

Note: Not a doctor nor does any of this qualify as medical advise. Follow at your own risk.

[FireLordSL]

Dear Warrior,

1.Do you have muscle weakening/muscle wasting and tiredness problems related to POIS?
If yes, is Nanna1's method helping to improve that?
2. So is there a progress in communication skills and social anxiety by now after following Nanna1 method ?
3. What is your progress of Nanna1 immune stack ?

[Warrior]

Dear Warrior,

1.Do you have muscle weakening/muscle wasting and tiredness problems related to POIS?
If yes, is Nanna1's method helping to improve that?
2. So is there a progress in communication skills and social anxiety by now after following Nanna1 method ?
3. What is your progress of Nanna1 immune stack ?

1. Yes, fatigue has always been one of my main POIS symptoms. This includes muscle weakness and tiredness. At one point, my fatigue during days 1-3 post ejaculation was incredibly intense. My original animal-based method made a world of a difference, but only as long as I stayed on the animal-based diet. Now with Nanna1's method, I can eat normally and I don't have any fatigue or muscle weakness.

2. So what I spoke about above is exactly the same for communication skills. My animal-based method allowed me to experience little to no social symptoms, as long as I stayed on animal-based. Now that Nanna1's method is working, I can eat normally, release, and socialise normally with zero communication symptoms. It's honestly crazy to me that this is real as I'm writing this.

3. I've temporarily paused the immune stack while I have been clarifying and tinkering with the POIS Cascade method. I plan on resuming it at some point in the near future.

Edit 18/01/24: I may still be experiencing some muscle fatigue. It's difficult to tell if it's the result of POIS/food sensitivity, or if it's because I'm not eating enough. I work a very physical job and often have a difficult time eating enough calories. In general, at this point in my life, with everything going on health-wise and the type of physical job I currently have, my body has been put under enormous physical stress. With that being said, if the method wasn't working at all, I would be experiencing extreme fatigue and muscle weakness.

[Warrior]

Importance of Vitamin D3 for POIS - you need more than you think! 2000iu of D3 isn't nearly enough!

Recently went back to 7000iu of D3 daily from 14,000iu and felt the slow and gradual increase of 'auditory thought noise' a classic symptom from my POIS. In the past I went from 14,000iu to 2000iu D3, and the increase in auditory thought noise was intense. This time round, it wasn't as intense, but still very bothersome. I have an indoor job, so my D3 supplement needs are naturally going to be higher than someone who spends their jobs outdoor. In the past, I used to work a labouring outdoor job (1.5 year ago) and got a tonne of sunlight. I got my D3 levels tested back then and they were solid. I took for granted how much D3 was helping my POIS back then, so have never spoken about how important D3 is, until recently in the past few months I have learnt how much worst POIS gets when your D3 are low.

I'm not a doctor so none of this is medical advice, but I do believe that if you have POIS, which is clearly some kind of inflammatory condition, your D3 needs are going to be higher than normal. Inflammatory conditions deplete D3 fast. I feel horrible on 2000iu/d of D3, I feel not as bad on 7000iu/d, and feel amazing on 14,000iu/d. You need to supplement vitamin K2 to prevent D3 toxicity risks with calcification. 100mcg of K2 per 10,000iu D3 is the general consensus recommendation. My functional medicine specialist recommended me 100mcg per 1000iu to be absolutely safe.

D3 must also be taken with a fatty meal as it's a fat soluble vitamin.

Dosages higher than 14,000iu/d may even be ideal - all depends on your D3 levels, absorption ability, and D3 needs. Getting D3 levels tested is recommended - listen to your body and health care specialist for guidance.

Great video
Another great video
https://youtu.be/tQ19ysKL8Nc?si=ZfwsrKOgLXBrcVaJ

Most doctors test pre-active form of Vitamin D. Must measure active form of Vitamin D for reliable measurement.
- Dr Berg also recommends 10,000iu-20,000iu as a safe maintence dosage. 100mcg K2 per 10,000iu D3.

[Warrior]

Update on the methyl (Nanna1's POIS Cascade Stack) stack:

Right now I'm using this stack (mostly the SAM-e and niacin) for utility purposes. I am yet to figure out a correct dosage and regime to prevent under and over-methylation permanently. I've had days where I can eat normal and experience zero POIS symptoms whatsoever. That's when the SAM-e is balanced. Then I have days where symptoms begin slipping through, until I take a corresponding under or over-methylated supplement (SAM-e or niacin.)

By utility this is what I mean:

START HERE: Experiencing POIS or food sensitivity symptoms ->
-> Am I under or over-methylated? -> I think I'm under, let's try SAM-e -> Feel better -> Fixed.
-> Am I under or over-methylated? -> I think I'm over, I've taken lots of SAM-e lately -> Try niacin -> Fixed.
-> Am I under or over-methylated? -> I think I'm under, let's try SAM-e -> Don't feel better / feel worst -> Try niacin -> Fixed.

I am yet to get the methyl buffer system working (glycine & Vitamin A). For now that is all theoretical. I haven't been able to prove it's effectiveness just yet. I don't seem to tolerate glycine very well, maybe I will try again in the future.

[Warrior]

I think it’s possible the monolaurin works in combination with other immune supplements. I tried testing it again the other day and didn’t really notice much impact on symptoms. I think something in Nanna1’s immune competence therapy or something in my additional supplements like Propolis has a synergistic effect with the monolaurin to inhibit POIS symptoms. It could be the case with biofilm disruptors, then enabling the monolaurin to penetrate. If anyone wants to experiment with monolaurin, an you have the money to do so, I would suggest the following complimentary supplements, most of which are from Nanna1's Immune Competence Therapy & a few others recommended by my specialist:

--Monolaurin
--Propolis 1-2 pills

May be cycled according to immune stimulation. From Nanna1's Immune Competence Therapy:
--AHCC 1-4 pills
--Beta-glucans 1g
--Andrographis 100mg (do not exceed, Monolaruin is already a potent antibiotic)

--Liposomal Vitamin C 1-2g
--Zinc 50mg
--Quercetin 2g

--Mega IgG2000 (biotoxin binder – take with food) 2-4x - optional, i took it at the time, so i'm listing it here, i doubt it would effect the monolaurin but i could be wrong.
--MegaSpore probiotic 1-2/d

[Progecitor]

I think it’s possible the monolaurin works in combination with other immune supplements. I tried testing it again the other day and didn’t really notice much impact on symptoms. I think something in Nanna1’s immune competence therapy or something in my additional supplements like Propolis has a synergistic effect with the monolaurin to inhibit POIS symptoms. It could be the case with biofilm disruptors, then enabling the monolaurin to penetrate. If anyone wants to experiment with monolaurin, an you have the money to do so, I would suggest the following complimentary supplements, most of which are from Nanna1's Immune Competence Therapy & a few others recommended by my specialist:

--Monolaurin
--Propolis 1-2 pills

May be cycled according to immune stimulation. From Nanna1's Immune Competence Therapy:
--AHCC 1-4 pills
--Beta-glucans 1g
--Andrographis 100mg (do not exceed, Monolaruin is already a potent antibiotic)

--Liposomal Vitamin C 1-2g
--Zinc 50mg
--Quercetin 2g

--Mega IgG2000 (biotoxin binder – take with food) 2-4x - optional, i took it at the time, so i'm listing it here, i doubt it would effect the monolaurin but i could be wrong.
--MegaSpore probiotic 1-2/d

It is really interesting that you mention monolaurin in conjunction with propolis. In my case the most significant benefit from both was a reduction of rhinitis. I have taken monolaurin for about a week, but I couldn’t notice much else otherwise. Maybe there was some reduction of dysuria as well. I have also tested some generic propolis recently, but it only had a lesser effect on rhinitis. However of all the supplements I have taken so far the Brazilian bee propolis trio was probably the most superior in the management of rhinitis. It did not solve my POIS in its entirety, but it is still a rather nice supplement. For this reason I would really recommend Brazilian bee propolis over a generic kind of propolis, though in my experience there is also a great variability between different brands, so one really needs to try many of them just to find one that works better.
By the way I have also taken SAM-e for about two weeks. There was a clear benefit on gut issues, which was about moderate in the beginning, but became a little less so later. However it did not help with depression or bloodshot eyes. It also did not resolve food sensitivities, as I could clearly feel the punch of POIS mimetics.

[Warrior]

I think it’s possible the monolaurin works in combination with other immune supplements. I tried testing it again the other day and didn’t really notice much impact on symptoms. I think something in Nanna1’s immune competence therapy or something in my additional supplements like Propolis has a synergistic effect with the monolaurin to inhibit POIS symptoms. It could be the case with biofilm disruptors, then enabling the monolaurin to penetrate. If anyone wants to experiment with monolaurin, an you have the money to do so, I would suggest the following complimentary supplements, most of which are from Nanna1's Immune Competence Therapy & a few others recommended by my specialist:

--Monolaurin
--Propolis 1-2 pills

May be cycled according to immune stimulation. From Nanna1's Immune Competence Therapy:
--AHCC 1-4 pills
--Beta-glucans 1g
--Andrographis 100mg (do not exceed, Monolaruin is already a potent antibiotic)

--Liposomal Vitamin C 1-2g
--Zinc 50mg
--Quercetin 2g

--Mega IgG2000 (biotoxin binder – take with food) 2-4x - optional, i took it at the time, so i'm listing it here, i doubt it would effect the monolaurin but i could be wrong.
--MegaSpore probiotic 1-2/d

It is really interesting that you mention monolaurin in conjunction with propolis. In my case the most significant benefit from both was a reduction of rhinitis. I have taken monolaurin for about a week, but I couldn’t notice much else otherwise. Maybe there was some reduction of dysuria as well. I have also tested some generic propolis recently, but it only had a lesser effect on rhinitis. However of all the supplements I have taken so far the Brazilian bee propolis trio was probably the most superior in the management of rhinitis. It did not solve my POIS in its entirety, but it is still a rather nice supplement. For this reason I would really recommend Brazilian bee propolis over a generic kind of propolis, though in my experience there is also a great variability between different brands, so one really needs to try many of them just to find one that works better.
By the way I have also taken SAM-e for about two weeks. There was a clear benefit on gut issues, which was about moderate in the beginning, but became a little less so later. However it did not help with depression or bloodshot eyes. It also did not resolve food sensitivities, as I could clearly feel the punch of POIS mimetics.

Thanks for sharing your experience of Brazillian bee propolis. I will have to look into that! I have often wondered about the quality of the generic propolis I have been taking from my local chemist warehouse lol

I will suggest one thing to you. You say SAM-e helped you a bit for gut issues at the beginning, but became less effective later on. I would suggest this is due to over-methylation from excess SAM-e. At the beginning, your SAM-e levels were most likely very depleted. Infections are known to deplete methyl groups (SAM-e.) There is a strong chance POIS is some kind of infection. Taking SAM-e did wonders for you until your body had enough methyl groups. Then the SAM-e pushed you into a state of over-methylation, and the original benefits from SAM-e deminished. Taking niacin 50mg will flush methyl groups, essentially doing the opposite of SAM-e. The trick would be to strike the correct balance of SAM-e. You may only need to supplement it once every 2nd day to prevent going into over-methylation. If you do go into over-methylation, take niacin 50mg every 1-2 hours until the original benefits from the SAM-e return.

This has at least been true for me in my direct experience in getting Nanna1's POIS Cascade stack to work again.

[Warrior]

Update 26/01/24. No POIS or food sensitivity when SAM-e is balanced. I've added 20,000iu D3 w/ K2 to my animal-based protocol seeing as how important that has been for my POIS and something I took for granted years ago when I was out in the sun all day. I eat animal-based throughout the day in case the SAM-e becomes unbalanced. I am still in the process of determining the most ideal SAM-e dosage and frequency for maintaining long-term balance.

Original Animal-Based Protocol:
--Paul Saladino’s Animal-Based Diet (Strictest Tier)
--Raw garlic ¼ clove, mince & sit for 5 mins before ingesting, take every 6 hrs
--Fenugreek 1-2g, take every 6 hrs
--Red meat 200-300g & eggs 2-3 per day
--Vitamin D3 20,000iu & K2 200mcg
--Nature’s Own Ultra B 150 Forte 1-2 per day. (Note: May interfere with SAM-e balance, especially nicotinamide. Long-term high-dose folic acid supplementation may also be of mild concern. Exploring other moderately dosed B complex’s with folinic acid. I am yet to find one that makes me feel as good as this one tho.)

POIS Cascade Stack (Leaks symptoms when SAM-e is unbalanced. No symptoms - no POIS or food sensitivities when SAM-e is balanced.):
--SAM-e 200mg active, taken on empty stomach

Find the right dosage frequency that maintains SAM-e balance long-term (neither under nor over-methylation):
1.   SAM-e 200mg 5 days on, 2 off, repeat
2.   SAM-e 200mg 4 days on, 1 off
3.   Alternatively, niacin 50mg every 1-2 hours will fix over-methylation (too much SAM-e), while taking more SAM-e will fix under-methylation (too little SAM-e)

--Choline (methyl donor) as 4 eggs

If I have zero social events planned for the evening:
1.   Re-feed window (in the evening) for non-Animal-Based carbs and other nutrient rich wholefoods which would typically cause symptoms when SAM-e isn’t balanced i.e., white rice, vegetables, and nuts.
2.   Fix any potential symptoms with a good night's sleep & supplementation (Niacin 50mg every 1-2 hours till feeling balanced, or SAM-e 200mg every 1-2 hours till feeling balanced – can also be taken the next morning.)

Beneficial Supplements to Cycle &/or Double Check for Negative Symptoms:
--EPA & DHA fish oil 1-2g and/or cod liver oil 1-2g
--Magnesium Citrate 300-400mg

Next step is to refocus on the immune stack which is a potential cure. Going to also post a video update on all of this soon.

[Progecitor]

Thanks for sharing your experience of Brazillian bee propolis. I will have to look into that! I have often wondered about the quality of the generic propolis I have been taking from my local chemist warehouse lol

I will suggest one thing to you. You say SAM-e helped you a bit for gut issues at the beginning, but became less effective later on. I would suggest this is due to over-methylation from excess SAM-e. At the beginning, your SAM-e levels were most likely very depleted. Infections are known to deplete methyl groups (SAM-e.) There is a strong chance POIS is some kind of infection. Taking SAM-e did wonders for you until your body had enough methyl groups. Then the SAM-e pushed you into a state of over-methylation, and the original benefits from SAM-e deminished. Taking niacin 50mg will flush methyl groups, essentially doing the opposite of SAM-e. The trick would be to strike the correct balance of SAM-e. You may only need to supplement it once every 2nd day to prevent going into over-methylation. If you do go into over-methylation, take niacin 50mg every 1-2 hours until the original benefits from the SAM-e return.

This has at least been true for me in my direct experience in getting Nanna1's POIS Cascade stack to work again.

Actually I tested niacin just the other day and it turned out to be a pleasant surprise. I have already taken a bigger box of niacinamide (500 mg/per pill), but it was not particularly useful. Now I bought the cheapest niacin (10 mg/per pill) of the same brand, thus probably about the same quality. I took 10, 20 and 30 mg of niacin along a day and had an O in the evening. Interestingly the first pill of niacin provided about the same benefit as a 500 mg of niacinamide pill would at least in the beginning. The second dose felt better and the third dose was the best. I took the last dose about an hour before the actual ejaculation. Now here comes the odd thing as on neither of these occasion occurred any flushing whatsoever and niacin was clearly useful regardless. The timing also does not work like for others as I was actually feeling a little worse after the O and niacin clearly follows the 6-7 hour rule as practically everything that I had taken so far. I was beginning to feel myself slightly better before going to bed which was about 3 hours after the last dose. However it was when I woke up during the night (7-8 hours after last dose) that I was feeling myself really well. It had a clear anti-depressive effect and by the light of the flashlight I could feel that even my photophobia was reduced, which hardly ever happens otherwise. In the morning (about 12 hours after the last dose) I was feeling myself a little worse and more like on a chronic morning, but even so it was still much better than I would expect on a first day. I am really glad that niacin is so useful and will surely implement it in a stack later. Later I may also try the 500 mg variety, but I don’t really want to take such a huge dose on a daily basis. I guess 1 mg/kg could be a safe dose, which is close to the amount I took on this occasion. I can only hope that its effectiveness will not plummet like with many of the other supplements that I had taken before, though based on others’ posts it is not likely.
At the moment I can’t say anything about synergies and I don’t know if there is any preference in taking SAM-e over other stuff as many of them provided a similar benefit (e.g. black cumin, cat’s claw etc.) with a cheaper price. Actually the current test also involved taking my usual daily and night stack which may have provided some synergies. The nightly stack involves resveratrol, fenugreek, garlic pills and some other stuff, but on other days they weren’t particularly effective otherwise.
By the way resveratrol acts as a DNMT1 and HDAC1 inhibitor and vitamin D as a DNMT1 inhibitor, thus they should reduce methylation. However this may also lead to vitamin D resistance.
https://poiscenter.com/forums/index.php?topic=4061.msg43890#msg43890
(ctrl+f: resveratrol or vitamin D)
Furthermore I have been taking a combination of fenugreek and garlic pills for about 5 month now, but they have never done anything significant. I bought two new brands of each besides the ones I had already tried, but no combination was any different. I have to note that I have only taken small doses though aside from the test period. The reason I take them is mostly a belief that they are generally healthy and not because they do any actual change. Currently I am testing several new stuff, but later I am going to gradually incorporate new supplements in the stack and then I may be able to tell more about niacin, SAM-e and the other stuff we had discussed previously.

[Warrior]

Little update 3/02/24. So it appears I have fixed the over-methylation problem by supplementing supporting B complex vitamins and removing nicotinamide. I stopped taking my B complex as it contained nicotinamide, known to remove methyl groups at high enough dosages, which isn't great given SAMe is part of this method. So I decided to buy the individual nutrients from the B complex so I could manipulate and control them better, seeing as the B complex always made me feel amazing. I've made a few different changes, so I don't entirely know what's responsible, apart from the fact that I don't seem to over-methylate anymore (or at least experience symptoms when taking too much SAMe), and seem to tolerate SAMe well, even 3x per day, while the method continues to work. It may also be that I've increased the dosage of SAMe cofactors, and perhaps I became deficient in them. I noticed significant improvement after increasing folic acid and B6, dosing twice a day.

This is currently what I'm taking:

Twice daily:
--SAMe 200mg 1-2x
--Methyl B12 1000mcg sublingual 1-2x
--Folic acid 1-2mg
--4 eggs worth of choline or equivalent via Choline Bitartrate
--B1 250mg
--B2 100mg
--B6 100mg [must be careful of toxicity]
--D3 20,000iu with K2 200mcg minimum, ideally more K2 (D3 dosed once per day with fatty meal)

—I will also mention red meat and eggs continue to make me feel amazing. I definitely prefer eggs as my choline source compared to choline bitartrate.

And these are the current tangible results:
--Virtually zero POIS symptoms. Can ejaculate as much as I want, and wake up feeling fresh the next day. Hasn't been a problem for a long time. My main focus these days are food sensitivities.
--Extremely improved food sensitivities. At times, virtually zero food sensitivity symptoms. Something I am still testing, and testing over the long haul. For example, I've eaten an entire sourdough pizza and ejaculated in the same night, and woken up with zero symptoms. I've eaten subway footlongs with gluten bread, which would normally (before this stack) cause an absolute outrage of symptoms like social anxiety, fatigue, depression, anxiety, brainfog, etc, and gone back to work, socialise, and feel great. I am still yet to go for long periods of time with zero symptoms after eating food, so the food sensitivity part is still a work in progress. But compared to where I've come from, it's a tremendous improvement nonetheless.
--I still get a small to moderate amount of muscle weakness from food sensitivities. Currently investigating whether adding Omega-3 can improve this by inhibiting AA cascade (as mentioned in Nanna1s theory + is a well known inflammatory pathway.) Also note, I work in a warehouse picking produce all day long, so this may also contribute to fatigue. Sometimes I pick heavy items like cases of 1kg carrots, pumpkin, etc. But that equally gives you a sense of how far I've come along. I used to get extreme fatigue from this condition, especially while eating a normal diet.
--I still get body aches which come and go, and appear at random parts of my body all over. Sometimes they can be intense, and definitely get worst when I deviate off an animal-based diet.

I'm still tinkering with this, but it's safe to say the SAM-e and methylation stack in general has worked for a while now (since discovering most of this from Nanna1.) I am just tinkering with the details to make it work perfectly. This is obviously bound to change as I make adjustments. Safe to say I totally recommend them to others here to experiment and play around with. I no longer even take raw garlic or fenugreek as this stack is so effective at removing all symptoms when it's functioning at it's prime.

Also a note on B vits, there are different kinds. Active i.e. methyl B12, methylfolate, and non-active i.e., cyanocobalamin (B12), folic acid. Depending on your genes, current methylation status, nutrients, and other factors, your body will prefer either active or non-active B vitamins. It's important to play around with this! Don't just assume after 1 B complex that it isn't for you because it gave you weird symptoms. Chances are 1 or 2 ingredients threw you out, while the others your body benefited from. Also play around with dosages. For a while I was taking no more than 0.5mg of folic acid per day. After discovering how important folate is for the methylation cycle, I realised I completely overlooked how important folate was, and after increasing the dosage, have felt a huge improvement.

Note: Not a doctor. Just a 23 year old dude, who's lived with this for about 6-7 years, done a mega tonne of self-experimentation and a decent amount of research. Just sharing what has worked for me, and reporting back here. I used to experience chronically intense anxiety, fatigue, body aches, social anxiety, weakness, brainfog, where the symptoms were so severe at one point I felt like I was going to get schizophrenia (my auditory thoughts became so loud from the chronic inflammation.) I talk about this on my YouTube channel.

[Warrior]

Continuing to have some really profound results on the methylation stack. Virtually zero POIS symptoms or food sensitivity symptoms.

In the past I spoke about the glycine buffer system, which would prevent over-methylation from excess SAM-e. When taking methyl donors of any kind, you are bound to take in more than what you currently need. If your buffer system isn't working, you will get a slew of over-methylation symptoms, which will actually trigger POIS symptoms. When your buffer system is working, your body will buffer the excess, prevent you from over-methylating, and store the excess for later retrieval when it needs it. So, after attempting supplementation of Vitamin A and glycine (two nutrients needed for the buffer system), I felt worse. It actually inhibited the SAM-e from working and I couldn't understand why.

It wasn't until I increased my folate supplementation that things actually begun working very smoothly. So in hindsight I actually believe I was deficient or very low in folate. Folate actually acts as the off-switch for the methyl-buffer system, which would explain why glycine and vitamin A inhibited the SAM-e from working, as there was not enough folate to turn the system off when it no longer needed to buffer methyl groups.

POIS inflammation depletes methyl groups. POIS mental symptoms are a result of depleted neurotransmitters, from depleted levels of SAMe. SAMe (S-Adenosyl-L-methionine) is required for the brain to synthesize the neurotransmitters norepinephrine, dopamine, and serotonin. SAMe is a universal methyl group donor. In addition to SAM-e, methyl B12, methylfolate (if tolerated), choline, and TMG (trimethylglycine) are all methyl donors, and should help to replace lost methyl groups from POIS, and thus help to fix POIS mental symptoms.

The catch is that you are working within a complex system which depends on many other nutrients to work properly. You also have different subtypes of nutrient supplementation (there are 3 main types of folate for example - folic acid, folinic acid, and methylfolate). For each nutrient, you must find the type and dosage that works well for you. Because we're all different, the chances are our methylation stacks will be slightly different. They need to be personalised.

With that being said, I would suggest all POIS sufferers to play around with the following supplements, which make up the methylation stack. You need to listen to your body, see what subtype of nutrients it likes, and at what dosages. As mentioned earlier, specific nutrients rely on adequate status of other nutrients to function properly. So it's possible that when you initially introduce certain supplements (SAM-e) for example, you may experience side-effects until you adequately satisfy it's co-factors.

Methylation Stack:
1. Important Methyl Cycle Nutrients: B12, Folate, B6, & Choline
2. Methyl Buffer System Nutrients: Glycine, Vitamin A, & Folate (which would be covered in step 1)
3. Extra Methylation Support for POIS: SAM-e, TMG, & Creatine

+ Vitamin D3 & K2. Immune issues always deplete D3, which is why many here improve when levels are optimal. Adequate K2 prevents D3 calcification side-effects. Vitamin A also gets depleted from immune problems. Another reason to supplement or at least ensure you have adequate amounts of it. Every immune event taxes both Vitamin A and Vitamin D. Zinc, Magnesium, Vitamin E and K2, all support fat-soluble vitamins. You must take fat-soluble vitamins with a fatty meal for absorption. You can learn more about this from Chris Masterjohn's website, specifically "Nutrition and Immunity Masterclass: Vitamins and Minerals in Immunity"

You may also not need to supplement everything listed here. For example, you may already have adequate vitamin A status, or TMG makes you feel funny while your body likes SAMe and other methyl donors. Choline is more important when you have low B12 and/or folate. SAM-e requires adequate folate and B12 levels, so if your body feels good after having choline, this may be a sign that you have low B12 and/or folate.

Methylation & Herpes Induced POIS:
Methyl-groups act as the off-switch for herpes virus.

Note: None of this constitutes as medical advice. I am not a doctor. Consult your medical specialists for medical advise. I am just sharing information from what I have learnt mostly from Chris Masterjohn Phd, which has worked very well for me in my direct experience.

[Andre2505]

Ciao Guerriero, sono il ragazzo che ti ha scritto recentemente su youtube sotto il tuo ultimo video. Bro io ho provato a prendere l aglio come hai detto tu ma mi ha lasciato comunque un cattivo alito per diverse ore nonostante ho mangiato e bevuto altre cose, ? difficile da mandare gi? per il suo sgradevole sapore, forse anche perch? avevo poco miele. Tu come fai a prenderlo senza alcuna difficolt? e senza che ti lasci un cattivo odore?