Post Orgasmic Illness Syndrome (P.O.I.S.)

General Category => Articles, References and Links => Topic started by: emi_b on May 30, 2011, 02:43:49 AM

Title: Peptide and Protein Constituents of Human Semen (2004)
Post by: emi_b on May 30, 2011, 02:43:49 AM
I've found a scientific article describing peptide and protein constituents of human semen.
An article (http://en.wikipedia.org/wiki/Antigen) from Wikipedia says that antigens are usually proteins or polysaccharides.

Article title: A comprehensive characterization of the peptide and protein constituents of human seminal fluid
Authors: Kim Y.C. Fung, L. Michael Glode, Spencer Green, Mark W. Duncan

Links:
     link 1 (http://onlinelibrary.wiley.com/doi/10.1002/pros.20089/abstract)

Quote
ABSTRACT
BACKGROUND
Knowledge of the peptide and protein components of seminal fluid and their role in prostate diseases including benign prostatic hyperplasia and prostate carcinoma is scant. We have undertaken a proteomic analysis of semen as a forerunner to identifying sensitive and specific diagnostic markers of prostatic diseases; to aid in improved therapeutic intervention; and, to enhance our understanding of prostate health and disease.

METHODS
Peptide and protein components of pooled human seminal fluid (n?=?5) were separated by gel electrophoresis (1D and 2D) and identified by either matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) or capillary liquid chromatography tandem mass spectrometry (LC–MS/MS).

RESULTS
Analysis by two-dimensional electrophoresis (2DE) established that there were multiple post-translational variants of the majority of the proteins. Hormones, growth factors and bioactive peptides were detected and identified.

CONCLUSIONS
We have identified over 100 protein and peptide components of normal human seminal fluid. © 2004 Wiley-Liss, Inc.